Kochia scoparia 열매 추출물이 구강 편평세포암종세포에서 예정된 괴사 유도
The Extract of Kochia scoparia Fruit Induces Programmed Necrosis in Oral Squamous Cell Carcinoma Cells
한혜련, 박봉수, 김형준, 정승화, 김지연, 정성희, 김규천, 황대석, 김욱규, 김형우, 류미현,
소속 상세정보
한혜련 ( Han Hye-Yeon ) - 부산대학교 치의학대학원 구강병리학교실
박봉수 ( Park Bong-Soo ) - 부산대학교 치의학대학원 구강해부학교실
김형준 ( Kim Hyung-Joon ) - 부산대학교 치의학대학원 구강생리학교실
정승화 ( Jeong Seung-Hwa ) - 부산대학교 치의학대학원 예방치과학교실
김지연 ( Kim Ji-Yeon ) - 부산대학교 치의학대학원 소아치과학교실
정성희 ( Jung Sung-Hee ) - 부산대학교 치의학대학원 구강진단학교실
김규천 ( Kim Gyoo-Cheon ) - 부산대학교 치의학대학원 구강해부학교실
황대석 ( Hwang Dae-Seok ) - 부산대학교 치의학대학원 구강악안면외과학교실
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치의학대학원 구강악안면외과학교실
김형우 ( Kim Hyung-Woo ) - 부산대학교 한의학대학원 약리학분과
류미현 ( Ryu Mi-Heon ) - BK21
KMID : 0829220160400060899
Abstract
The fruit of Kochia scoparia Scharder is traditionally used as a medicinal ingredient to treat allergic skin diseases and inflammatory diseases in China, Japan and Korea. Recently, several studies reported that K. scoparia had potential for the cytotoxicity of human cancer cells. To investigate the anti-cancer effect of K. scoparia on oral cancer and to determine the specific type of cell death induced by MEKS treatment. We investigated the anti-cancer effects of K. scoparia, methanol extract (MEKS) in HSC4 human oral cancer cells. We examined the effects of MEKS on the proliferation rate, cell cycle arrest, 7-AAD-ANNEXIN V double stain, reactive oxygen species (ROS) generation and activation of apoptosis and necroptosis-associated proteins in HSC4 cells. MTT assay results demonstrated that MEKS decreased the proliferation rates of HSC4 cells in a dose-dependent manner with an IC50 value of 45.3 μg/ml. MEKS at 50 μg/ml significantly increased the sub-G1 DNA contents of HSC4 cells to 84.8%, versus untreated cells. However, the activation of apoptosis-associated proteins such as cleaved caspase 3, cleaved caspase 8, cleaved caspase 9 and cleaved Poly (ADP-ribose) polymerase (PARP) did not detect. The level of Bax protein markedly increased in MEKS-treated HSC4 cells. In addition, the cell viability of the DPQ pre-treated HSC4 cells with MEKS treatment was significantly greater than that of MEKS treated-cells. These results suggest that MEKS inhibits cell proliferation and induces necroptosis in oral cancer cells and that MEKS may have potential chemotherapeutic value for the treatment of human oral cancer.
키워드
Necroptosis; oral cancer; Kochia scoparia; reactive oxygen species
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